RT-PCR COVID-19 Test Kit

RT-PCR COVID-19 Test Kit

AAL LS COVID-19 Test is an in vitro diagnostic real-time RT-PCR test intended for the qualitative detection of nucleic acid from SARS-CoV-2 in nasopharyngeal, oropharyngeal, mid-turbinate, and anterior nasal swabs collected from individuals suspected of COVID-19 by their healthcare provider.

The LS COVID-19 kit is ICMR Approved and is intended for use by qualified and trained clinical laboratory personnel specifically instructed in the techniques of real-time PCR and in vitro diagnostic procedures.

Kit components (100 rxns/box)

The reagents included in one kit are sufficient for testing 100 samples

  Name Volumes Quantity Storage upon receipt
1 Positive Control 10 μL 1 - 20 ℃
2 RT-PCR Master Mix 625 μL 1 - 20 ℃
3 Multiplex Pool 75 μL 1 - 20 ℃
4 RNase Free H2O 100 μL 1 - 20 ℃

ORF1 ab gene +N gene

The LS COVID-19 is a multiplex real-time reverse transcription polymerase chain reaction test (real-time RT-PCR). The SARS-CoV-2 Primer and Probe sets are designed to detect RNA of the 2019-SARS nCoV in upper respiratory specimens from patients suspected of COVID-19 disease by their healthcare provider.

The nucleic acid sequences of primers and probes and their target genes used in the test are listed in the following table.

Table 1. Primer and probe sets and target genes

Target Fluorescent dye Quencher
ORF1ab FAM None
N HEX None
RNAse P (Internal Control) ROX None