Next Generation Sequencing

It is a technology for determining the sequence of DNA or RNA to study genetic variation associated with diseases or other biological phenomena.

We offer following platform to provide sequencing services:

Illumina

PacBio

Oxford Nanopore

Roche

We provide wide variety of services including:

Metagenomics

Whole Exome Sequencing

Whole Genome Sequencing

Transcriptomics

Methylation Sequencing

ChIP Sequencing

Small RNA Sequencing

Plasmid Sequencing

Viral Genome Sequencing

Targeted Resequencing

Bioinformatics

Requirement: DNA and Primer Information

Sanger Sequencing upto 700 bp (unidirectional)

Sanger Sequencing upto 1500 bp (bidirectional, contiq can be provided in .txt)

Obligate anaerobes (We need only DNA for this kind of Organism)

It is the study of microbes in their natural living environment, which involves the complex microbial communities in which they usually exist. The study examines the genomic composition of an entire organism, including each of the microbes that exist within it. It is an important concept for the microbes and the host to be thought of as interdependent and observed as a community, rather than considered to be separate entities.

Whole-exome sequencing is a widely used Next Generation Sequencing (NGS) method that involves sequencing the protein-coding regions of the genome. The human exome represents less than 2% of the genome, but contains ~85% of known disease-related variants, making this method a cost-effective alternative to whole-genome sequencing.

Exome sequencing using exome enrichment can efficiently identify coding variants across a broad range of applications, including population genetics, genetic disease, and cancer studies.

Whole-genome sequencing (WGS) is a comprehensive method for analyzing entire genomes. Genomic information has been instrumental in identifying inherited disorders, characterizing the mutations that drive cancer progression, and tracking disease outbreaks. Rapidly dropping sequencing costs and the ability to produce large volumes of data with today’s sequencers make whole-genome sequencing a powerful tool for genomics research. While this method is commonly associated with sequencing human genomes, the scalable, flexible nature of next-generation sequencing (NGS) technology makes it equally useful for sequencing any species, such as agriculturally important livestock, plants, or disease-related microbes.

The study of all the RNA molecules within a cell. Transcriptome focus on messenger RNA molecules only, which reflect the genes that are being actively expressed (as protein products) in a cell or tissue at a given time or in a given situation. However, over 95% of the RNAs in a cell are not translated into a protein, so transcriptomics also includes the study of these non-coding RNAs, which have a dizzying variety of forms and functions.

It is used to study, epigenetic markers. Bisulfite sequencing is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation.

ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.

Small RNA sequencing is a type of RNA sequencing based on the use of NGS technologies that allows to isolate and get information about noncoding RNA molecules in order to evaluate and discover new forms of small RNA and to predict their possible functions.

Plasmid sequencing allows the identification of the genetic changes behind bacterial plasmid adaptation to new hosts or habitats as well as the co-evolution of bacterial genomes and plasmids during the introduction of new genes.

Virus genome sequencing is contribute to the tracking of epidemics and the development of new methods of control.

Targeted resequencing is a method that aims to identify causative mutations within discrete genomic loci in populations by isolating and sequencing genomic regions of interest from a sample library.